Dataset Information

The clearance of cell remnants and the regeneration of the injured muscle depend on soluble pattern recognition receptor PTX3.

ABSTRACT:

Objective

The signals causing the resolution of muscle inflammation are only partially characterized. The long pentraxin PTX3, which modulates leukocyte recruitment and activation, could contribute.

Methods

We analysed the expression of ptx3 after muscle injury and verified whether hematopoietic precursors are a source of the protein. The kinetics of regeneration and leukocytes infiltration, the accumulation of cell remnants and anti-histidyl-t-RNA synthetase autoantibodies were compared in wild-type and ptx3-deficient mice.

Results

Ptx3 expression was up-regulated three-five days after injury and restricted to the extracellular matrix. Cellular debris and leukocytes persisted in the muscle of ptx3-deficient mice for a long time after wild-type animals had healed. ptx3-deficient macrophages expressed receptors involved in apoptotic cell clearance and engulfed dead cells in vitro. Accumulation of cell debris in a pro-inflammatory microenvironment was not sufficient to elicit autoantibodies.

Conclusion

PTX3 generated in response to muscle injury prompts the clearance of debris and the termination of the inflammatory response.

SUBMITTER: Vezzoli M

PROVIDER: S-EPMC5263058 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Publications

The clearance of cell remnants and the regeneration of the injured muscle depend on soluble pattern recognition receptor PTX3.

Vezzoli Michela M Sciorati Clara C Campana Lara L Monno Antonella A Doglio Maria Giulia MG Rigamonti Elena E Corna Gianfranca G Touvier Thierry T Castiglioni Alessandra A Capobianco Annalisa A Mantovani Alberto A Manfredi Angelo A AA Garlanda Cecilia C Rovere-Querini Patrizia P

Molecular medicine (Cambridge, Mass.) 20161123

<h4>Objective</h4>The signals causing the resolution of muscle inflammation are only partially characterized. The long pentraxin PTX3, which modulates leukocyte recruitment and activation, could contribute.<h4>Methods</h4>We analysed the expression of ptx3 after muscle injury and verified whether hematopoietic precursors are a source of the protein. The kinetics of regeneration and leukocytes infiltration, the accumulation of cell remnants and anti-histidyl-t-RNA synthetase autoantibodies were c ...[more]

PMID: 27900389

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Dataset Information

The clearance of cell remnants and the regeneration of the injured muscle depend on soluble pattern recognition receptor PTX3.

Objective

Methods

Results

Conclusion

Publications

The clearance of cell remnants and the regeneration of the injured muscle depend on soluble pattern recognition receptor PTX3.

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