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Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGF? Signaling in Rat Anterior Pituitary Gland.

ABSTRACT: Fibromodulin belongs to the family of small leucine-rich proteoglycans (SLRPs), an active component of extracellular matrix. It directly binds collagens to promote fibrillogenesis and also binds transforming growth factor-beta (TGF?) to antagonize its actions. Our previous studies of rat anterior pituitary gland revealed that fibromodulin is expressed in folliculostellate cells and pericytes. Although our recent study showed that TGF?2 secreted from folliculostellate cells induces collagen synthesis in pericytes, the involvement of fibromodulin in TGF?2-mediated collagen regulation has not been studied. The present study examined the effect of TGF?2 on fibromodulin synthesis in rat anterior pituitary gland. In situ hybridization for TGF? receptor II and immunohistological techniques revealed the presence of TGF? receptor II in folliculostellate cells and pericytes. To confirm canonical TGF? intracellular signaling, Smad2 immunocytochemistry was performed. Nuclear translocation of Smad2 was observed in folliculostellate cells and pericytes after TGF?2 treatment. TGF?2 strongly enhanced fibromodulin mRNA and protein expressions, and TGF?2-induced mRNA expression was completely blocked by TGF? receptor I inhibitor (SB431542). These results suggest that folliculostellate cells and pericytes exhibit canonical TGF?2 signaling, which is associated with fibromodulin production. Thus, this is the first report to show that TGF? signaling regulates the endogenous TGF? antagonist fibromodulin in the gland.

SUBMITTER: Syaidah R

PROVIDER: S-EPMC5263227 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGFβ Signaling in Rat Anterior Pituitary Gland.

Syaidah Rahimi R Tsukada Takehiro T Azuma Morio M Horiguchi Kotaro K Fujiwara Ken K Kikuchi Motoshi M Yashiro Takashi T

Acta histochemica et cytochemica 20161222 6

Fibromodulin belongs to the family of small leucine-rich proteoglycans (SLRPs), an active component of extracellular matrix. It directly binds collagens to promote fibrillogenesis and also binds transforming growth factor-beta (TGFβ) to antagonize its actions. Our previous studies of rat anterior pituitary gland revealed that fibromodulin is expressed in folliculostellate cells and pericytes. Although our recent study showed that TGFβ2 secreted from folliculostellate cells induces collagen synth ...[more]

PMID: 28127105

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Project description:Folliculostellate cell gap junctions establish a network for the transmission of information within the anterior pituitary. Connexins make up gap junction channels. Changes in connexin (Cx) turnover modify gap junction-mediated intercellular communication. We have reported that cytokines and hormones influence Cx43 turnover and coupling in folliculostellate cells and in the folliculostellate cell line TtT/GF. In addition, the expression of different connexins alters intercellular communication and connexins may have functions besides cell coupling. Here we assessed the expression, turnover and subcellular localization of Cx46 and Cx50 in the anterior pituitary and TtT/GF cells. Then, we assessed the impact of various natural (lactation, annual reproductive cycle, bFGF) and pathological (autoimmune orchitis, diabetes/obesity) conditions associated with altered anterior pituitary hormone secretion on Cx46 and Cx50. Anterior pituitary Cx46 and Cx50 expression and subcellular distribution were cell-dependent. Cx46 was expressed by folliculostellate, TtT/GF and endocrine cells. In the cytoplasm, Cx46 was chiefly associated with lysosomes. Variously sized Cx46 molecules were recovered exclusively in the TtT/GF cell nuclear fraction. In the nucleus, Cx46 co-localized with Nopp-140, a nucleolar factor involved in rRNA processing. Neither cytoplasmic nor nuclear Cx46 and Cx43 co-localized. Cx50 localized to folliculostellate and TtT/GF cells, and to the walls of blood capillaries, not to endocrine cells. Cx50 was cytoplasmic and associated with the cell membrane, not nuclear. Cx50 did not co-localize with Cx46 but it co-localized in the cytoplasm and co-immunoprecipitated with Cx43. Cx46 and Cx50 responses to various physiological and pathological challenges were different, often opposite. Cx46 and Cx43 expression and phosphorylation profiles differed in the anterior pituitary, whereas Cx50 and Cx43 were similar. The data suggest that Cx46 participates to cellular growth and proliferation and that Cx50, together with Cx43, contributes to folliculostellate cell coupling.

Dataset Information

Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGF? Signaling in Rat Anterior Pituitary Gland.

Publications

Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGFβ Signaling in Rat Anterior Pituitary Gland.

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