Dataset Information

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn²⁺ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

ABSTRACT: Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn²⁺) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5?nm) for 12?h exhibited significant decrease (23?±?8.7%) in % mitotic index and increase in chromosomal aberrations (18?±?7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ??m were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO₂^- at 1240?cm^-1 and stretching of C-O ribose at 1060?cm^-1, suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn²⁺ in plants.

SUBMITTER: Ahmed B

PROVIDER: S-EPMC5264391 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Publications

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn<sup>2+</sup> Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

Ahmed Bilal B Dwivedi Sourabh S Abdin Malik Zainul MZ Azam Ameer A Al-Shaeri Majed M Khan Mohammad Saghir MS Saquib Quaiser Q Al-Khedhairy Abdulaziz A AA Musarrat Javed J

Scientific reports 20170125

Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn<sup>2+</sup>) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited s ...[more]

PMID: 28120857

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Project description:Appropriate effluent treatment processes are expected to significantly reduce the toxicity of effluents before they are released to the natural environment. The present study was aimed to assess the spatial and temporal variations of the physical and chemical water quality parameters of a natural water body receiving treated textile effluents and to assess the chromosomal abnormalities induced by the treated textile effluents. Four sampling sites (A: effluent discharge point; B: 100?m downstream from site A along the tributary; C: 200?m downstream from site A along the tributary; D: 100?m upstream from site A along the tributary) were selected associated to a tributary that received treated textile effluent. The physical and chemical water quality parameters were measured in the composite water samples collected from the study sites, and Allium cepa bioassay was conducted using aged tap water as the control. Sampling was conducted in both rainy and dry seasons. The conductivity, TDS, COD, and colour intensity of the water samples collected from the study sites were significantly higher during the dry season compared to those in the rainy season. Allium cepa root meristematic cells exposed to water samples from sites A, B, and C showed a significantly high interphase and prophase indices compared to those exposed to aged tap water and upstream site during both rainy and dry seasons. The mitotic index of the root tip cells of Allium cepa bulbs exposed to the water samples collected from the effluent discharge point (site A) and from the 100?m downstream site from site A (site B) was significantly lower than that of the other sites in both rainy and dry seasons. However, the mitotic index of the root tip cells of Allium cepa bulbs exposed to the water samples from the upstream site was not significantly different from that of the control treatment during both sampling seasons. The bioassay indicated that the mitotic index and phase index of the root meristematic cells of Allium cepa can be affected by the treated textile effluents released to the water body and the occurrence of C metaphase, chromosomal adherence, bridges, disturbed anaphase, vagrant chromosomes, and chromosomal breaks indicated that the treated textile effluent receiving tributary can possibly contain genotoxic and mutagenic compounds which can induce chromosomal abnormalities.

Dataset Information

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn²⁺ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

Publications

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn<sup>2+</sup> Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

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Dataset Information

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

Publications

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn<sup>2+</sup> Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn²⁺ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.