Dataset Information

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition.

ABSTRACT:

Background

The role of platelet-rich plasma (PRP) in the treatment of sport-related injuries is unclear, largely due to the heterogeneity of clinical results. This may relate to compositional differences in PRP from different separation systems.

Questions/purposes

This study aims to compare the composition of PRP produced with five different commercially available systems, focusing on cellular concentrations and pH.

Methods

Seven donors (41?±?12 years) provided blood for PRP preparation using five systems (Arthrex Angel, Emcyte Genesis CS, Arteriocyte Magellan, Harvest SmartPrep, and Biomet GPS III). Post processing, cellular composition was measured including platelets (PLT), white blood cells (WBC), neutrophils (NE), and red blood cells (RBC), as well as pH.

Results

Platelet concentration and capture efficiency were similar between systems, except the Angel 7% preparation had a greater concentration than Genesis CS (2310?±?524 vs. 1129?±?264 k/?L). WBC concentration was variable between systems; however, significant differences were only found between the Angel 2% and GPS III preparations (11.0?±?4.5, 27.3?±?7.1 k/?L). NE concentration was significantly lower in the Angel 2% and 7% preparations compared with GPS III (0.6?±?0.6 and 1.8?±?1.3 k/?L vs. 9.4?±?7.0 k/?L). RBC concentration was highest in SmartPrep (3.2?±?0.6 M/?L) and Genesis CS systems (3.1?±?0.6 M/?L) compared with all other systems (?1.1?±?1.2 M/?L). Finally, pH was significantly lower with the SmartPrep system (6.95?±?0.06) compared with all others (?7.26?±?0.06).

Conclusion

Aside from platelet concentration and capture efficiency, significant compositional differences were identified between preparation systems. Caution should be employed when interpreting clinical results of studies utilizing PRP, as the role of compositional differences and their effect on outcome are unknown. Further study is necessary to determine the clinical significance of these differences.

SUBMITTER: Degen RM

PROVIDER: S-EPMC5264574 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Publications

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition.

Degen Ryan M RM Bernard Johnathan A JA Oliver Kristin S KS Dines Joshua S JS

HSS journal : the musculoskeletal journal of Hospital for Special Surgery 20160819 1

<h4>Background</h4>The role of platelet-rich plasma (PRP) in the treatment of sport-related injuries is unclear, largely due to the heterogeneity of clinical results. This may relate to compositional differences in PRP from different separation systems.<h4>Questions/purposes</h4>This study aims to compare the composition of PRP produced with five different commercially available systems, focusing on cellular concentrations and pH.<h4>Methods</h4>Seven donors (41 ± 12 years) provided blood for PR ...[more]

PMID: 28167878

Similar Datasets

Project description:BACKGROUND:Carprofen and platelet-rich plasma (PRP) are widely used in small animal clinical practice. Separation layers have been used during blood centrifugation to increase platelet yield. The objectives of this study were to (1) identify the optimal centrifugation force for the one-step PRP preparation, (2) determine whether there is an advantage to using carprofen in one-step PRP preparation, and (3) compare platelet morphology from one-step PRP preparation with and without carprofen. We hypothesized that injectable carprofen (emulsion formula) could be used successfully as the separation layer in PRP preparation. RESULTS:Samples from 14 healthy dogs were used to determine the optimal centrifugation force using one-step PRP preparation in a disposable syringe without carprofen, with forces set at 300, 500, 700, 900, 1100, 1300, and 1500 xg for 5?min. Optimum centrifugation force, plasma volume, and platelet concentrations of one-step PRP preparation were found and recovered at 900 xg, 1.9?±?0.28?ml, and 260.50?±?58.39 X 103 cell/?l, respectively. Samples from 12 healthy dogs were used to determine the optimal force (with forces set at 300, 500, 700, and 900 xg) for 5?min using one-step PRP preparation with carprofen. Optimum centrifugation force, plasma volume, and platelet concentrations for one-step PRP preparation with carprofen were found and recovered at 500 xg, 0.62?±?0.16?ml and 948.50?±?261.40 X 103 cell/?l, respectively. One-step PRP preparation with carprofen increased the platelet yield from baseline by 1.76 and 4.95 fold, respectively. Samples from 3 healthy dogs were used to observe platelet morphologies after centrifugation by scanning electron microscopy. Images of platelets on glass slides from both preparation methods revealed pseudopods emerging from the margins of the discoid platelets. CONCLUSIONS:One-step PRP centrifugation both with and without carprofen increased the platelet yield, but using carprofen (emulsion formula) as a separation layer resulted in a higher platelet yield. The clinical usefulness of PRP products from these methods should be further investigated.

Dataset Information

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition.

Background

Questions/purposes

Methods

Results

Conclusion

Publications

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition.

Similar Datasets

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