Dataset Information

Technical Procedures for Preparation and Administration of Platelet-Rich Plasma and Related Products: A Scoping Review.

ABSTRACT:

Introduction

Platelet-rich plasma is widely used for different types of clinical situations, but universal standardization of procedures for its preparation is still lacking.

Methods

Scoping review of comparative studies that have assessed at least two alternatives in one or more stages of preparation, storage and/or administration of PRP or its related products. A systematic search was conducted in MEDLINE, Embase, and LILACS. Two authors screened references independently. Data extraction was performed iteratively, and results were presented for each included comparison.

Results

Thirty-nine studies were included after assessing full texts, focusing on the comparison of PRP to a related product, types of anticoagulants, centrifugation protocols, commercial kits, processing time, methods for activation, and application concomitantly to other substances. Only laboratory outcomes were assessed, as platelet, leukocyte and growth factor concentrations.

Conclusion

Results showed great variability related to methods employed in different stages of PRP processing, which may explain the variability observed in clinical trials assessing the efficacy of PRP for different clinical situations.

SUBMITTER: Pachito DV

PROVIDER: S-EPMC7759516 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Technical Procedures for Preparation and Administration of Platelet-Rich Plasma and Related Products: A Scoping Review.

Pachito Daniela Vianna DV Bagattini Ângela Maria ÂM de Almeida Adriano Marques AM Mendrone-Júnior Alfredo A Riera Rachel R

Frontiers in cell and developmental biology 20201211

<h4>Introduction</h4>Platelet-rich plasma is widely used for different types of clinical situations, but universal standardization of procedures for its preparation is still lacking.<h4>Methods</h4>Scoping review of comparative studies that have assessed at least two alternatives in one or more stages of preparation, storage and/or administration of PRP or its related products. A systematic search was conducted in MEDLINE, Embase, and LILACS. Two authors screened references independently. Data e ...[more]

PMID: 33363154

Similar Datasets

Project description:BackgroundThe role of platelet-rich plasma (PRP) in the treatment of sport-related injuries is unclear, largely due to the heterogeneity of clinical results. This may relate to compositional differences in PRP from different separation systems.Questions/purposesThis study aims to compare the composition of PRP produced with five different commercially available systems, focusing on cellular concentrations and pH.MethodsSeven donors (41 ± 12 years) provided blood for PRP preparation using five systems (Arthrex Angel, Emcyte Genesis CS, Arteriocyte Magellan, Harvest SmartPrep, and Biomet GPS III). Post processing, cellular composition was measured including platelets (PLT), white blood cells (WBC), neutrophils (NE), and red blood cells (RBC), as well as pH.ResultsPlatelet concentration and capture efficiency were similar between systems, except the Angel 7% preparation had a greater concentration than Genesis CS (2310 ± 524 vs. 1129 ± 264 k/μL). WBC concentration was variable between systems; however, significant differences were only found between the Angel 2% and GPS III preparations (11.0 ± 4.5, 27.3 ± 7.1 k/μL). NE concentration was significantly lower in the Angel 2% and 7% preparations compared with GPS III (0.6 ± 0.6 and 1.8 ± 1.3 k/μL vs. 9.4 ± 7.0 k/μL). RBC concentration was highest in SmartPrep (3.2 ± 0.6 M/μL) and Genesis CS systems (3.1 ± 0.6 M/μL) compared with all other systems (≤1.1 ± 1.2 M/μL). Finally, pH was significantly lower with the SmartPrep system (6.95 ± 0.06) compared with all others (≥7.26 ± 0.06).ConclusionAside from platelet concentration and capture efficiency, significant compositional differences were identified between preparation systems. Caution should be employed when interpreting clinical results of studies utilizing PRP, as the role of compositional differences and their effect on outcome are unknown. Further study is necessary to determine the clinical significance of these differences.

Dataset Information

Technical Procedures for Preparation and Administration of Platelet-Rich Plasma and Related Products: A Scoping Review.

Introduction

Methods

Results

Conclusion

Publications

Technical Procedures for Preparation and Administration of Platelet-Rich Plasma and Related Products: A Scoping Review.

Similar Datasets

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