Ontology highlight
ABSTRACT:
SUBMITTER: Foley TL
PROVIDER: S-EPMC5269537 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Foley Timothy L TL Dorjsuren Dorjbal D Dexheimer Thomas S TS Burkart Michael D MD Wight William C WC Simeonov Anton A
Journal of biomolecular screening 20160710 10
We describe a polyacrylamide gel casting cassette that overcomes limitations of commercially available gel electrophoresis equipment. This apparatus molds a single polyacrylamide gel that can evaluate more than 200 samples in parallel, is loaded with a multichannel pipettor, and is flexible with respect to composition of the separating matrix. We demonstrate its use to characterize inhibitors of enzymes that modify protein and nucleic acid substrates. Throughputs of greater than 1000 samples per ...[more]