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Viral interference of the bacterial RNA metabolism machinery.

ABSTRACT: In a recent publication, we reported a unique interaction between a protein encoded by the giant myovirus phiKZ and the Pseudomonas aeruginosa RNA degradosome. Crystallography, site-directed mutagenesis and interactomics approaches revealed this 'degradosome interacting protein' or Dip, to adopt an 'open-claw' dimeric structure that presents acidic patches on its outer surface which hijack 2 conserved RNA binding sites on the scaffold domain of the RNase E component of the RNA degradosome. This interaction prevents substrate RNAs from being bound and degraded by the RNA degradosome during the virus infection cycle. In this commentary, we provide a perspective into the biological role of Dip, its structural analysis and its mysterious evolutionary origin, and we suggest some therapeutic and biotechnological applications of this distinctive viral protein.

SUBMITTER: Dendooven T

PROVIDER: S-EPMC5270514 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Viral interference of the bacterial RNA metabolism machinery.

Dendooven Tom T Van den Bossche An A Hendrix Hanne H Ceyssens Pieter-Jan PJ Voet Marleen M Bandyra K J KJ De Maeyer Marc M Aertsen Abram A Noben Jean-Paul JP Hardwick Steven W SW Luisi Ben F BF Lavigne Rob R

RNA biology 20161111 1

In a recent publication, we reported a unique interaction between a protein encoded by the giant myovirus phiKZ and the Pseudomonas aeruginosa RNA degradosome. Crystallography, site-directed mutagenesis and interactomics approaches revealed this 'degradosome interacting protein' or Dip, to adopt an 'open-claw' dimeric structure that presents acidic patches on its outer surface which hijack 2 conserved RNA binding sites on the scaffold domain of the RNase E component of the RNA degradosome. This ...[more]

PMID: 27834591

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