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From guide to target: molecular insights into eukaryotic RNA-interference machinery.

ABSTRACT: Since its relatively recent discovery, RNA interference (RNAi) has emerged as a potent, specific and ubiquitous means of gene regulation. Through a number of pathways that are conserved in eukaryotes from yeast to humans, small noncoding RNAs direct molecular machinery to silence gene expression. In this Review, we focus on mechanisms and structures that govern RNA silencing in higher organisms. In addition to highlighting recent advances, we discuss parallels and differences among RNAi pathways. Together, the studies reviewed herein reveal the versatility and programmability of RNA-induced silencing complexes and emphasize the importance of both upstream biogenesis and downstream silencing factors.

SUBMITTER: Ipsaro JJ

PROVIDER: S-EPMC4450863 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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From guide to target: molecular insights into eukaryotic RNA-interference machinery.

Ipsaro Jonathan J JJ Joshua-Tor Leemor L

Nature structural & molecular biology 20150101 1

Since its relatively recent discovery, RNA interference (RNAi) has emerged as a potent, specific and ubiquitous means of gene regulation. Through a number of pathways that are conserved in eukaryotes from yeast to humans, small noncoding RNAs direct molecular machinery to silence gene expression. In this Review, we focus on mechanisms and structures that govern RNA silencing in higher organisms. In addition to highlighting recent advances, we discuss parallels and differences among RNAi pathways ...[more]

PMID: 25565029

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