Synchronized purification and immobilization of his-tagged ?-glucosidase via Fe3O4/PMG core/shell magnetic nanoparticles.

ABSTRACT: In this paper, an efficient and convenient Fe3O4/PMG/IDA-Ni2+ nanoparticles that applied to purify and immobilize his-tagged ?-glucosidase was synthesized, in which, Fe3O4/PMG (poly (N, N'-methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni2+. The gene of ?-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a frequently used method in the field of purifying his-tagged proteins from crude cell lysates. The results indicated that Fe3O4/PMG/IDA-Ni2+ nanoparticles can be considered as an excellent purification material. ?-glucosidase was immobilized on the surface of Fe3O4/PMG/IDA-Ni2+ to form Fe3O4/PMG/IDA-?-glucosidase by means of covalent bound with imidazolyl and Ni2+. The immobilized ?-glucosidase exhibited excellent catalytic activity and stabilities compared with free ?-glucosidase. In addition, immobilized ?-glucosidase can be recycled for many times and retain more than 65% of the original activity. The materials display enormous potential in the aspect of purifying and immobilizing enzyme.

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC5278371 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature