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Ultra-fast cell counters based on microtubular waveguides.

ABSTRACT: We present a radio-frequency impedance-based biosensor embedded inside a semiconductor microtube for the in-flow detection of single cells. An impedance-matched tank circuit and a tight wrapping of the electrodes around the sensing region, which creates a close, leakage current-free contact between cells and electrodes, yields a high signal-to-noise ratio. We experimentally show a twofold improved sensitivity of our three-dimensional electrode structure to conventional planar electrodes and support these findings by finite element simulations. Finally, we report on the differentiation of polystyrene beads, primary mouse T lymphocytes and Jurkat T lymphocytes using our device.

SUBMITTER: Bausch CS

PROVIDER: S-EPMC5278506 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Ultra-fast cell counters based on microtubular waveguides.

Bausch Cornelius S CS Heyn Christian C Hansen Wolfgang W Wolf Insa M A IM Diercks Björn-Philipp BP Guse Andreas H AH Blick Robert H RH

Scientific reports 20170130

We present a radio-frequency impedance-based biosensor embedded inside a semiconductor microtube for the in-flow detection of single cells. An impedance-matched tank circuit and a tight wrapping of the electrodes around the sensing region, which creates a close, leakage current-free contact between cells and electrodes, yields a high signal-to-noise ratio. We experimentally show a twofold improved sensitivity of our three-dimensional electrode structure to conventional planar electrodes and supp ...[more]

PMID: 28134293

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