Dataset Information

Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.

ABSTRACT:

Introduction

Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases.

Purpose

The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using ?-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds.

Methods

Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale.

Results

oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model.

Conclusions

The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies.

SUBMITTER: Sanjurjo-Rodriguez C

PROVIDER: S-EPMC5283731 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Publications

Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.

Sanjurjo-Rodríguez Clara C Castro-Viñuelas Rocío R Hermida-Gómez Tamara T Fernández-Vázquez Tania T Fuentes-Boquete Isaac Manuel IM de Toro-Santos Francisco Javier FJ Díaz-Prado Silvia María SM Blanco-García Francisco Javier FJ

PloS one 20170131 1

<h4>Introduction</h4>Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases.<h4>Purpose</h4>The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds.<h4>Methods</h4>Cells isolated from ovine bone ma ...[more]

PMID: 28141815

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Dataset Information

Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.

Introduction

Purpose

Methods

Results

Conclusions

Publications

Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.

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