Ontology highlight
ABSTRACT:
SUBMITTER: Huang SN
PROVIDER: S-EPMC5286372 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Huang Shar-Yin N SN Williams Jessica S JS Arana Mercedes E ME Kunkel Thomas A TA Pommier Yves Y
The EMBO journal 20161208 3
Ribonuclease activity of topoisomerase I (Top1) causes DNA nicks bearing 2',3'-cyclic phosphates at ribonucleotide sites. Here, we provide genetic and biochemical evidence that DNA double-strand breaks (DSBs) can be directly generated by Top1 at sites of genomic ribonucleotides. We show that RNase H2-deficient yeast cells displayed elevated frequency of Rad52 foci, inactivation of RNase H2 and RAD52 led to synthetic lethality, and combined loss of RNase H2 and RAD51 induced slow growth and repli ...[more]