Ontology highlight
ABSTRACT:
SUBMITTER: Wu J
PROVIDER: S-EPMC5291289 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Wu Jing J Zhu Jianhui J Yin Haidi H Liu Xinhua X An Mingrui M Pudlo Nicholas A NA Martens Eric C EC Chen Grace Y GY Lubman David M DM
Journal of proteome research 20160902 10
Metaproteomics is one approach to analyze the functional capacity of the gut microbiome but is limited by the ability to evenly extract proteins from diverse organisms within the gut. Herein, we have developed a pipeline to optimize sample preparation of stool obtained from germ-free (GF) mice that were gavaged a defined community of 11 bacterial strains isolated from the human gut. With 64% more proteins identified, bead-beating was confirmed to be an indispensable step for the extraction of ba ...[more]