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Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.

ABSTRACT: Severe xerostomia (dry mouth) compromises the quality of life in patients with Sjögren's syndrome or radiation therapy for head and neck cancer. A clinical management of xerostomia is often unsatisfactory as most interventions are palliative with limited efficacy. Following up our previous study demonstrating that mouse BM-MSCs are capable of differentiating into salivary epithelial cells in a co-culture system, we further explored the molecular basis that governs the MSC reprogramming by utilizing high-throughput iTRAQ-2D-LC-MS/MS-based proteomics. Our data revealed the novel induction of pancreas-specific transcription factor 1a (PTF1?), muscle, intestine and stomach expression-1 (MIST-1), and achaete-scute complex homolog 3 (ASCL3) in 7 day co-cultured MSCs but not in control MSCs. More importantly, a common notion of pancreatic-specific expression of PTF1 ? was challenged for the first time by our verification of PTF1 ? expression in the mouse salivary glands. Furthermore, a molecular network simulation of our selected putative MSC reprogramming factors demonstrated evidence for their perspective roles in salivary gland development. In conclusion, quantitative proteomics with extensive data analyses narrowed down a set of MSC reprograming factors potentially contributing to salivary gland regeneration. Identification of their differential/synergistic impact on MSC conversion warrants further investigation.

SUBMITTER: Park YJ

PROVIDER: S-EPMC5291466 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.

Park Yun-Jong YJ Koh Jin J Kwon Jin Teak JT Park Yong-Seok YS Yang Lijun L Cha Seunghee S

PloS one 20170203 2

Severe xerostomia (dry mouth) compromises the quality of life in patients with Sjögren's syndrome or radiation therapy for head and neck cancer. A clinical management of xerostomia is often unsatisfactory as most interventions are palliative with limited efficacy. Following up our previous study demonstrating that mouse BM-MSCs are capable of differentiating into salivary epithelial cells in a co-culture system, we further explored the molecular basis that governs the MSC reprogramming by utiliz ...[more]

PMID: 28158262

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Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.

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Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.

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