SERS-Based Flavonoid Detection Using Ethylenediamine-?-Cyclodextrin as a Capturing Ligand.

ABSTRACT: Ethylenediamine-modified ?-cyclodextrin (Et-?-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO?@Ag@Et-?-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-?-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO?@Ag@Et-?-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10^-7 to 10^-3 M than various organic molecules, namely ethylene glycol, ?-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO?@Ag@Et-?-CD NPs remained even after repeated washing. These results indicated that the SiO?@Ag@Et-?-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.

SUBMITTER: Choi JM 

PROVIDER: S-EPMC5295198 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature