Ontology highlight
ABSTRACT:
SUBMITTER: Noguera ME
PROVIDER: S-EPMC5299410 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Noguera Martín E ME Vazquez Diego S DS Ferrer-Sueta Gerardo G Agudelo William A WA Howard Eduardo E Rasia Rodolfo M RM Manta Bruno B Cousido-Siah Alexandra A Mitschler André A Podjarny Alberto A Santos Javier J
Scientific reports 20170209
Thioredoxin is a ubiquitous small protein that catalyzes redox reactions of protein thiols. Additionally, thioredoxin from E. coli (EcTRX) is a widely-used model for structure-function studies. In a previous paper, we characterized several single-point mutants of the C-terminal helix (CTH) that alter global stability of EcTRX. However, spectroscopic signatures and enzymatic activity for some of these mutants were found essentially unaffected. A comprehensive structural characterization at the at ...[more]