Ontology highlight
ABSTRACT:
SUBMITTER: Bailey JK
PROVIDER: S-EPMC5300091 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Bailey Jennifer K JK Nguyen Dung N DN Horiya Satoru S Krauss Isaac J IJ
Tetrahedron 20160729 40
Recently, we reported a directed evolution method which enabled us to discover sequences of glycopeptides that bind with picomolar affinity to HIV antibody 2G12 and are of interest as HIV vaccine candidates. In this manuscript, we describe the syntheses of several of these large (~11-12 kDa) glycopeptides by a combination of fast flow peptide synthesis and click chemistry. We also discuss the optimization of their attachment to carrier protein CRM197, affording antigenic and immunogenic conjugat ...[more]