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Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.

ABSTRACT: Live imaging has become a powerful tool in studies of ciliary proteins. Tetrahymena thermophila is an established ciliated model with well-developed genetic and biochemical approaches, but its large size, complex shape, and the large number of short and overlapping cilia, have made live imaging of ciliary proteins challenging. Here we describe a method that combines paralysis of cilia by nickel ions and total internal reflection microscopy for live imaging of fluorescent proteins inside cilia of Tetrahymena. Using this method, we quantitatively documented the intraflagellar transport in Tetrahymena.

SUBMITTER: Jiang YY

PROVIDER: S-EPMC5301313 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.

Jiang Yu-Yang YY Lechtreck Karl K Gaertig Jacek J

Methods in cell biology 20150214

Live imaging has become a powerful tool in studies of ciliary proteins. Tetrahymena thermophila is an established ciliated model with well-developed genetic and biochemical approaches, but its large size, complex shape, and the large number of short and overlapping cilia, have made live imaging of ciliary proteins challenging. Here we describe a method that combines paralysis of cilia by nickel ions and total internal reflection microscopy for live imaging of fluorescent proteins inside cilia of ...[more]

PMID: 25837403

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