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Single-shot super-resolution total internal reflection fluorescence microscopy.

ABSTRACT: We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.

SUBMITTER: Guo M

PROVIDER: S-EPMC7470603 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Single-shot super-resolution total internal reflection fluorescence microscopy.

Guo Min M Chandris Panagiotis P Giannini John Paul JP Trexler Adam J AJ Fischer Robert R Chen Jiji J Vishwasrao Harshad D HD Rey-Suarez Ivan I Wu Yicong Y Wu Xufeng X Waterman Clare M CM Patterson George H GH Upadhyaya Arpita A Taraska Justin W JW Shroff Hari H

Nature methods 20180507 6

We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface. ...[more]

PMID: 29735999

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