Ontology highlight
ABSTRACT:
SUBMITTER: Mangiameli SM
PROVIDER: S-EPMC5305214 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Mangiameli Sarah M SM Merrikh Christopher N CN Wiggins Paul A PA Merrikh Houra H
eLife 20170116
The canonical model of DNA replication describes a highly-processive and largely continuous process by which the genome is duplicated. This continuous model is based upon <i>in vitro</i> reconstitution and <i>in vivo</i> ensemble experiments. Here, we characterize the replisome-complex stoichiometry and dynamics with single-molecule resolution in bacterial cells. Strikingly, the stoichiometries of the replicative helicase, DNA polymerase, and clamp loader complexes are consistent with the presen ...[more]