Dataset Information

The effect of renal dysfunction and haemodialysis on circulating liver specific miR-122.

ABSTRACT:

Aims

microRNA-122 (miR-122) is a hepatotoxicity biomarker with utility in the management of paracetamol overdose and in drug development. Renal dysfunction and haemodialysis have been associated with a reduction in circulating microRNA. The objective of this study was to determine their effect on miR-122.

Methods

Blood samples were collected from 17 patients with end-stage renal disease (ESRD) on haemodialysis, 22 healthy controls, 30 patients with chronic kidney disease (CKD) and 15 patients post-kidney transplantation. All had normal standard liver function tests. Samples from ESRD patients were collected immediately pre- and post-haemodialysis. Serum alanine transaminase activity (ALT), miR-122 and miR-885 (liver enriched) were compared.

Results

Circulating miR-122 was substantially reduced in ESRD patients pre-haemodialysis compared with the other groups (19.0-fold lower than healthy controls; 21.7-fold lower than CKD). Haemodialysis increased miR-122 from a median value of 6.7 × 10³ (2.3 × 10³ -1.4 × 10⁴ ) to 1.6 × 10⁴ (5.4 × 10³ -3.2 × 10⁴ ) copies ml^-1 . The increase in miR-122 did not correlate with dialysis adequacy. miR-122 was reduced in the argonaute 2 bound fraction pre-haemodialysis; this fraction was increased post-dialysis. There was no change in miR-122 associated with extracellular vesicles. miR-885 was also reduced in ESRD patients (4-fold compared to healthy subjects) and increased by haemodialysis.

Conclusion

miR-122 is substantially lower in ESRD compared to healthy controls, patients with CKD and transplanted patients. Haemodialysis increases the concentration of miR-122. These data need to be considered when interpreting liver injury using miR-122 in patients with ESRD on dialysis, and specific reference ranges that define normal in this setting may need to be developed.

SUBMITTER: Rivoli L

PROVIDER: S-EPMC5306495 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Publications

The effect of renal dysfunction and haemodialysis on circulating liver specific miR-122.

Rivoli Laura L Vliegenthart A D Bastiaan AD de Potter Carmelita M J CM van Bragt Job J M H JJ Tzoumas Nikolaos N Gallacher Peter P Farrah Tariq E TE Dhaun Neeraj N Dear James W JW

British journal of clinical pharmacology 20161021 3

<h4>Aims</h4>microRNA-122 (miR-122) is a hepatotoxicity biomarker with utility in the management of paracetamol overdose and in drug development. Renal dysfunction and haemodialysis have been associated with a reduction in circulating microRNA. The objective of this study was to determine their effect on miR-122.<h4>Methods</h4>Blood samples were collected from 17 patients with end-stage renal disease (ESRD) on haemodialysis, 22 healthy controls, 30 patients with chronic kidney disease (CKD) and ...[more]

PMID: 27650800

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Dataset Information

The effect of renal dysfunction and haemodialysis on circulating liver specific miR-122.

Aims

Methods

Results

Conclusion

Publications

The effect of renal dysfunction and haemodialysis on circulating liver specific miR-122.

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