Dataset Information

Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.

ABSTRACT: Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs. We describe cell-type- and patient-specific transcriptional hierarchies. Single-cell transcriptomics on cultured live adult patient derived cells is a prime example of the promise of personalized precision medicine. Because these cells derive from subjects ranging in age into their sixties, this system permits human aging studies previously possible only in rodent systems.

SUBMITTER: Spaethling JM

PROVIDER: S-EPMC5316103 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.

Spaethling Jennifer M JM Na Young-Ji YJ Lee Jaehee J Ulyanova Alexandra V AV Baltuch Gordon H GH Bell Thomas J TJ Brem Steven S Chen H Isaac HI Dueck Hannah H Fisher Stephen A SA Garcia Marcela P MP Khaladkar Mugdha M Kung David K DK Lucas Timothy H TH O'Rourke Donald M DM Stefanik Derek D Wang Jinhui J Wolf John A JA Bartfai Tamas T Grady M Sean MS Sul Jai-Yoon JY Kim Junhyong J Eberwine James H JH

Cell reports 20170101 3

Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequ ...[more]

PMID: 28099855

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Dataset Information

Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.

Publications

Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.

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