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Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents.

ABSTRACT: Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparable or a slightly higher delivery efficiency of mRNA compared to the initial TT3 LLNs. Moreover, a strong MRI signal was observed in the cell pellets treated with TT3-Gd18 LLNs. More importantly, TT3-Gd18 LLNs demonstrated an efficient delivery of mRNA and Gd contrast agents in vivo.

SUBMITTER: Luo X

PROVIDER: S-EPMC5316423 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents.

Luo X X Li B B Li B B Zhang X X Zhao W W Bratasz A A Deng B B McComb D W DW Dong Y Y

Nanoscale 20170101 4

Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparab ...[more]

PMID: 28067926

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