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Three-dimensional histology: new visual approaches to morphological changes during neural regeneration.


ABSTRACT: Three-dimensional (3D) histology utilizes tissue clearing techniques to turn intact tissues transparent, allowing rapid interrogation of tissue architecture in three dimensions. In this article, we summarized the available tissue clearing methods and classified them according to their physicochemical principles of operation, which provided a framework for one to choose the best techniques for various research settings. Recent attempts in addressing various questions regarding the degenerating and regenerating nervous system have been promising with the use of 3D histological techniques.

SUBMITTER: Lai HM 

PROVIDER: S-EPMC5319234 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Three-dimensional histology: new visual approaches to morphological changes during neural regeneration.

Lai Hei Ming HM   Ng Ho Man HM   Wu Wutian W  

Neural regeneration research 20170101 1


Three-dimensional (3D) histology utilizes tissue clearing techniques to turn intact tissues transparent, allowing rapid interrogation of tissue architecture in three dimensions. In this article, we summarized the available tissue clearing methods and classified them according to their physicochemical principles of operation, which provided a framework for one to choose the best techniques for various research settings. Recent attempts in addressing various questions regarding the degenerating an  ...[more]

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