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Vitamin C enhances the expression of IL17 in a Jmjd2-dependent manner.

ABSTRACT: Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in CD4?Foxp3? regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes. Rather, vitamin C reduced the trimethylation of histone H3 lysine 9 (H3K9me3) in the regulatory elements of the Il17 locus, and the effects of vitamin C were abrogated by knockdown of jumonji-C domain-containing protein 2 (jmjd2). These results suggest that vitamin C can affect the expression of IL17 by modulating the histone demethylase activity. [BMB Reports 2017; 50(1): 49-54].

SUBMITTER: Song MH

PROVIDER: S-EPMC5319665 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Vitamin C enhances the expression of IL17 in a Jmjd2-dependent manner.

Song Mi Hye MH Nair Varun Sasidharan VS Oh Kwon Ik KI

BMB reports 20170101 1

Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in CD4＋Foxp3＋ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interesti ...[more]

PMID: 27931518

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