Dataset Information

High-throughput DNA sequencing of the moose rumen from different geographical locations reveals a core ruminal methanogenic archaeal diversity and a differential ciliate protozoal diversity.

ABSTRACT: Moose rumen samples from Vermont, Alaska and Norway were investigated for methanogenic archaeal and protozoal density using real-time PCR, and diversity using high-throughput sequencing of the 16S and 18S rRNA genes. Vermont moose showed the highest protozoal and methanogen densities. Alaskan samples had the highest percentages of Methanobrevibacter smithii, followed by the Norwegian samples. One Norwegian sample contained 43?% Methanobrevibacter thaueri, whilst all other samples contained 70?% Polyplastron multivesiculatum and one contained >75?% Entodinium spp. Protozoa from Norwegian moose belonged predominantly (>50?%) to the genus Entodinium, especially Entodinium caudatum. Norwegian moose contained a large proportion of sequences (25-97?%) which could not be classified beyond family. Protozoa from Vermont samples were predominantly Eudiplodinium rostratum (>75?%), with up to 7?% Diploplastron affine. Four of the eight Vermont samples also contained 5-12?% Entodinium spp. Samples were heterogeneous based on AMOVA, principal coordinate analysis and UniFrac. This study gives the first insight into the methanogenic archaeal diversity in the moose rumen. The high percentage of rumen archaeal species associated with high starch diets found in Alaskan moose corresponds well to previous data suggesting that they feed on plants high in starch. Similarly, the higher percentage of species related to forage diets in Vermont moose also relates well to their higher intake of fibre.

SUBMITTER: Ishaq SL

PROVIDER: S-EPMC5320624 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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High-throughput DNA sequencing of the moose rumen from different geographical locations reveals a core ruminal methanogenic archaeal diversity and a differential ciliate protozoal diversity.

Ishaq Suzanne L SL Sundset Monica A MA Crouse John J Wright André-Denis G AG

Microbial genomics 20151030 4

Moose rumen samples from Vermont, Alaska and Norway were investigated for methanogenic archaeal and protozoal density using real-time PCR, and diversity using high-throughput sequencing of the 16S and 18S rRNA genes. Vermont moose showed the highest protozoal and methanogen densities. Alaskan samples had the highest percentages of <i>Methanobrevibacter smithii</i>, followed by the Norwegian samples. One Norwegian sample contained 43 % <i>Methanobrevibacter thaueri</i>, whilst all other samples c ...[more]

PMID: 28348818

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Dataset Information

High-throughput DNA sequencing of the moose rumen from different geographical locations reveals a core ruminal methanogenic archaeal diversity and a differential ciliate protozoal diversity.

Publications

High-throughput DNA sequencing of the moose rumen from different geographical locations reveals a core ruminal methanogenic archaeal diversity and a differential ciliate protozoal diversity.

Similar Datasets

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