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Measuring Oxygen Consumption Rate in Caenorhabditis elegans.


ABSTRACT: The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.

SUBMITTER: Palikaras K 

PROVIDER: S-EPMC5321503 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Measuring Oxygen Consumption Rate in <i>Caenorhabditis elegans</i>.

Palikaras Konstantinos K   Tavernarakis Nektarios N  

Bio-protocol 20161201 23


The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras <i>et al.</i>, 2015; Ryu <i>et al.</i>, 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen <i>et al.</i>, 2015; Vander Heiden <i>et al.</i>, 2009). In this protocol, we describe a method for the det  ...[more]

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