Ontology highlight
ABSTRACT:
SUBMITTER: Musante L
PROVIDER: S-EPMC5328348 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Musante Luca L Tataruch-Weinert Dorota D Kerjaschki Dontscho D Henry Michael M Meleady Paula P Holthofer Harry H
Journal of extracellular vesicles 20161223 1
Urinary extracellular vesicles (UEVs) appear an ideal source of biomarkers for kidney and urogenital diseases. The majority of protocols designed for their isolation are based on differential centrifugation steps. However, little is still known of the type and amount of vesicles left in the supernatant. Here we used an isolation protocol for UEVs which uses hydrostatic filtration dialysis as first pre-enrichment step, followed by differential centrifugation. Transmission electron microscopy (TEM ...[more]