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AttI1-Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence.

ABSTRACT: By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within the attI1 site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (blaGES-1) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression.

SUBMITTER: Papagiannitsis CC

PROVIDER: S-EPMC5328530 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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attI1-Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence.

Papagiannitsis Costas C CC Tzouvelekis Leonidas S LS Tzelepi Eva E Miriagou Vivi V

Antimicrobial agents and chemotherapy 20170223 3

By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within the attI1 site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (blaGES-1) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression. ...[more]

PMID: 28031195

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