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Automatic Quantification of the Number of Intracellular Compartments in Arabidopsis thaliana Root Cells.

ABSTRACT: In the era of quantitative biology, it is increasingly required to quantify confocal microscopy images. If possible, quantification should be performed in an automatic way, in order to avoid bias from the experimenter, to allow the quantification of a large number of samples, and to increase reproducibility between laboratories. In this protocol, we describe procedures for automatic counting of the number of intracellular compartments in Arabidopsis root cells, which can be used for example to study endocytosis or secretory trafficking pathways and to compare membrane organization between different genotypes or treatments. While developed for Arabidopsis roots, this method can be used on other tissues, cell types and plant species.

SUBMITTER: Bayle V

PROVIDER: S-EPMC5330412 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Automatic Quantification of the Number of Intracellular Compartments in Arabidopsis thaliana Root Cells.

Bayle Vincent V Platre Matthieu Pierre MP Jaillais Yvon Y

Bio-protocol 20170201 4

In the era of quantitative biology, it is increasingly required to quantify confocal microscopy images. If possible, quantification should be performed in an automatic way, in order to avoid bias from the experimenter, to allow the quantification of a large number of samples, and to increase reproducibility between laboratories. In this protocol, we describe procedures for automatic counting of the number of intracellular compartments in Arabidopsis root cells, which can be used for examp ...[more]

PMID: 28255574

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