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Quantification of Starch in Guard Cells of Arabidopsis thaliana.

ABSTRACT: In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.

SUBMITTER: Flutsch S 

PROVIDER: S-EPMC6046211 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Quantification of Starch in Guard Cells of <i>Arabidopsis thaliana</i>.

Flütsch Sabrina S   Distefano Luca L   Santelia Diana D  

Bio-protocol 20180701 13

In this protocol, we describe how to quantify starch in guard cells of <i>Arabidopsis thaliana</i> using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells. ...[more]

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