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Versatile synthesis of the signaling peptide glorin.

ABSTRACT: We present a versatile synthesis of the eukaryotic signaling peptide glorin as well as glorinamide, a synthetic analog. The ability of these compounds to activate glorin-induced genes in the social amoeba Polysphondylium pallidum was evaluated by quantitative reverse transcription PCR, whereby both compounds showed bioactivity comparable to a glorin standard. This synthetic route will be useful in conducting detailed structure-activity relationship studies as well as in the design of chemical probes to dissect glorin-mediated signaling pathways.

SUBMITTER: Barnett R

PROVIDER: S-EPMC5331329 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Versatile synthesis of the signaling peptide glorin.

Barnett Robert R Raszkowski Daniel D Winckler Thomas T Stallforth Pierre P

Beilstein journal of organic chemistry 20170208

We present a versatile synthesis of the eukaryotic signaling peptide glorin as well as glorinamide, a synthetic analog. The ability of these compounds to activate glorin-induced genes in the social amoeba <i>Polysphondylium pallidum</i> was evaluated by quantitative reverse transcription PCR, whereby both compounds showed bioactivity comparable to a glorin standard. This synthetic route will be useful in conducting detailed structure-activity relationship studies as well as in the design of chem ...[more]

PMID: 28326133

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