Ontology highlight
ABSTRACT:
SUBMITTER: Kamau E
PROVIDER: S-EPMC5331890 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20170105
<h4>Background</h4>Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses.<h4>Objectives</h4>Establish if mismatches in the primer and probe binding sites could have reduced real-time RT-PCR sensitivity.<h4>Study design</h4>Nucleoprotein (N) and glycoprotein (G) genes were sequenced for real-time RT-PCR positive and negat ...[more]