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Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution.


ABSTRACT: We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing.

SUBMITTER: Cho EH 

PROVIDER: S-EPMC5339101 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution.

Cho Eun Hye EH   Park Kyung Sun KS   Yang Mina M   Song Dong Joon DJ   Huh Hee Jae HJ   Ki Chang Seok CS   Lee Nam Yong NY  

Annals of laboratory medicine 20170501 3


We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level.  ...[more]

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