Unknown

Dataset Information

0

Genetic Dissection of a QTL Affecting Bone Geometry.


ABSTRACT: Parameters of bone geometry such as width, length, and cross-sectional area are major determinants of bone strength. Although these traits are highly heritable, few genes influencing bone geometry have been identified. Here, we dissect a major quantitative trait locus (QTL) influencing femur size. This QTL was originally identified in an F2 cross between the C57BL/6J-hg/hg (HG) and CAST/EiJ strains and was referred to as femur length in high growth mice 2 (Feml2). Feml2 was located on chromosome (Chr.) 9 at ∼20 cM. Here, we show that the HG.CAST-(D9Mit249-D9Mit133)/Ucd congenic strain captures Feml2 In an F2 congenic cross, we fine-mapped the location of Feml2 to an ∼6 Mbp region extending from 57.3 to 63.3 Mbp on Chr. 9. We have identified candidates by mining the complete genome sequence of CAST/EiJ and through allele-specific expression (ASE) analysis of growth plates in C57BL/6J × CAST/EiJ F1 hybrids. Interestingly, we also find that the refined location of Feml2 overlaps a cluster of six independent genome-wide associations for human height. This work provides the foundation for the identification of novel genes affecting bone geometry.

SUBMITTER: Sabik OL 

PROVIDER: S-EPMC5345717 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genetic Dissection of a QTL Affecting Bone Geometry.

Sabik Olivia L OL   Medrano Juan F JF   Farber Charles R CR  

G3 (Bethesda, Md.) 20170310 3


Parameters of bone geometry such as width, length, and cross-sectional area are major determinants of bone strength. Although these traits are highly heritable, few genes influencing bone geometry have been identified. Here, we dissect a major quantitative trait locus (QTL) influencing femur size. This QTL was originally identified in an F2 cross between the C57BL/6J-hg/hg (HG) and CAST/EiJ strains and was referred to as femur length in high growth mice 2 (<i>Feml2</i>). <i>Feml2</i> was located  ...[more]

Similar Datasets

2018-10-15 | E-MTAB-1434 | biostudies-arrayexpress
| S-EPMC5661080 | biostudies-literature
| PRJEB1248 | ENA
| S-EPMC3394711 | biostudies-literature
2016-10-07 | GSE87712 | GEO
| S-EPMC4893171 | biostudies-literature
| S-EPMC3345892 | biostudies-literature
2015-10-01 | GSE70531 | GEO
2016-10-07 | GSE87711 | GEO
| S-EPMC8495944 | biostudies-literature