IFN-? Displays Anti-Inflammatory Effects on Staphylococcus aureus Endometritis via Inhibiting the Activation of the NF-?B and MAPK Pathways in Mice.

ABSTRACT: The aim of the present study was to determine the anti-inflammatory effect of IFN-? on endometritis using a mouse model of S. aureus-induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN-? on S. aureus growth was monitored by turbidimeter at 600 nm. IFN-? did not affect S. aureus growth. The histopathological changes indicated that IFN-? had a protective effect on uterus tissues with S. aureus infection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-?, IL-1?, and IL-6 was decreased with IFN-? treatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-?. Furthermore, the western blotting results showed the phosphorylation of I?B, NF-?B p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN-? treatment. The results suggested that IFN-? may be a potential drug for the treatment of uterine infection due to S. aureus or other infectious inflammatory diseases.

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC5346370 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature