Ontology highlight
ABSTRACT:
SUBMITTER: Ioannidou K
PROVIDER: S-EPMC5347081 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Ioannidou Kalliopi K Baumgaertner Petra P Gannon Philippe O PO Speiser Michel F MF Allard Mathilde M Hebeisen Michael M Rufer Nathalie N Speiser Daniel E DE
Scientific reports 20170313
The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay, with titrated amounts of peptide. Using this assay, we developed a method revealing the heterogeneity of functional avidity, represented by the steepness/hillslope of the peptide t ...[more]