Dataset Information

Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair.

ABSTRACT:

Aim

Progressive ischemia due to peripheral artery disease causes muscle damage and reduced strength of the lower extremities. Autologous cell therapy is an attractive treatment to restore perfusion and improve muscle function. Adipose-derived stem cells (ASCs) have therapeutic potential in tissue repair, including polarizing effects on macrophages (MPs).

Materials & methods

Co-culture systems of ASCs and MPs were analyzed for gene and protein expression modifications in ASC-conditioned MPs. Co-transplantation of MPs/ASCs in vivo led to improved skeletal muscle regeneration in a mouse model of peripheral artery disease.

Results

ASCs/MPs therapy restored muscle function, increased perfusion and reduced inflammatory infiltrate.

Conclusion

Combined MPs/ASCs cell therapy is a promising approach to restore muscle function and stimulate local angiogenesis in the ischemic limb.

SUBMITTER: Rybalko V

PROVIDER: S-EPMC5348723 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Publications

Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair.

Rybalko Viktoriya V Hsieh Pei-Ling PL Ricles Laura M LM Chung Eunna E Farrar Roger P RP Suggs Laura J LJ

Regenerative medicine 20170216 2

<h4>Aim</h4>Progressive ischemia due to peripheral artery disease causes muscle damage and reduced strength of the lower extremities. Autologous cell therapy is an attractive treatment to restore perfusion and improve muscle function. Adipose-derived stem cells (ASCs) have therapeutic potential in tissue repair, including polarizing effects on macrophages (MPs).<h4>Materials & methods</h4>Co-culture systems of ASCs and MPs were analyzed for gene and protein expression modifications in ASC-condit ...[more]

PMID: 28244825

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Project description:Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neuronal death and infiltration of inflammatory cells, exerting a neuroprotective effect. We hypothesized that the combination of mild therapeutic hypothermia and adipose-derived stem cells would be neuroprotective for treatment of stroke. A rat model of transient middle cerebral artery occlusion was established using the nylon monofilament method. Mild therapeutic hypothermia (33°C) was induced after 2 hours of ischemia. Adipose-derived stem cells were administered through the femoral vein during reperfusion. The severity of neurological dysfunction was measured by a modified Neurological Severity Score Scaling System. The area of the infarct lesion was determined by 2,3,5-triphenyltetrazolium chloride staining. Apoptotic neurons were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. The regeneration of microvessels and changes in the glial scar were detected by immunofluorescence staining. The inflammatory responses after ischemic brain injury were evaluated by in situ staining using markers of inflammatory cells. The expression of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction. Compared with mild therapeutic hypothermia or adipose-derived stem cell treatment alone, their combination substantially improved neurological deficits and decreased infarct size. They synergistically reduced the number of TUNEL-positive cells and glial fibrillary acidic protein expression, increased vascular endothelial growth factor levels, effectively reduced inflammatory cell infiltration and down-regulated the mRNA expression of the proinflammatory cytokines interleukin-1?, tumor necrosis factor-? and interleukin-6. Our findings indicate that combined treatment is a better approach for treating stroke compared with mild therapeutic hypothermia or adipose-derived stem cells alone.

Dataset Information

Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair.

Aim

Materials & methods

Results

Conclusion

Publications

Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair.

Similar Datasets

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