Ontology highlight
ABSTRACT:
SUBMITTER: Smith MS
PROVIDER: S-EPMC535139 | biostudies-literature | 2004 Dec
REPOSITORIES: biostudies-literature
Smith Marilyn S MS Yang Richard K RK Knapp Charles W CW Niu Yafen Y Peak Nicholas N Hanfelt Margery M MM Galland John C JC Graham David W DW
Applied and environmental microbiology 20041201 12
A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tcr) genes [tet(O), tet(W), and tet(Q)] in mixed microbial communities resident in feedlot lagoon wastewater. Tcr gene real-time TaqMan primer-probe sets were developed and optimized to quantify the Tcr genes present in seven different cattle feedlot lagoons, to validate the method, and to assess whether resistance gene concentrations correlate with free-tetracycline levels in lagoon waters. The me ...[more]