Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations.

ABSTRACT: Heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a Ca(OH)2-enriched, extremely diluted groundwater with pH 11.4. One hundred eighty-five strains were isolated in different media at different pH values during two sampling periods. To assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the isolates by using random amplified polymorphic DNA-PCR profiles and grouped them based on similarities determined by fatty acid methyl ester analysis. Phenotypic characterization, determinations of G+C content, phylogenetic analyses by direct sequencing of 16S rRNA genes, and determinations of pH tole

SUBMITTER: Tiago I 

PROVIDER: S-EPMC535156 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature