Bacterial rapid adaptation to alkaline environments
Ontology highlight
ABSTRACT:
ORGANISM(S): Bacillus thuringiensis
PROVIDER: GSE270261 | GEO | 2024/06/24
REPOSITORIES: GEO
Similar Datasets
Project description:Bacterial rapid adaptation to alkaline environments
| PRJNA1125898 | ENA
Project description:This project contributes to the knowledge of the proteomic data of each of 24 microbial strains grown in its most favorable condition in aerobiosis condition and harvested at stationary phase. The bacteria used are Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Bacillus thuringiensis DSM 5815, Bordetella parapertussis Bpp5, Cellulophaga lytica DSM 7489, Deinococcus deserti VCD115, Deinococcus geothermalis DSM 11300, Deinococcus proteolyticus DSM 20540, Kineococcus radiotolerans SRS30216, Marivirga tractuosa DSM 4126, Oceanibulbus indolifex HEL-45, Oceanicola granulosus HTCC2516, Phaeobacter inhibens DSM 17395, Pseudomonas putida mt-2 KT2440, Pseudopedobacter saltans DSM 12145, Roseobacter denitrificans OCh 114, Roseovarius nubinhibens ISM, Ruegeria pomeroyi DSS-3, Sagittula stellata E 37, Salmonella bongori NCTC 12419, Shigella flexneri 2a 2457T, Sphingomonas wittichii RW1, Staphylococcus carnosus TM300, Vibrio harveyi ATCC 14126
2023-06-02 | PXD005728 | Pride
Project description:Transcriptional profiling of C. elegans nasp-1 / btr-1 mutant worms versus wild type N2 strain, both exposed to the bacterial pathogen Bacillus thuringiensis DB27. One-condition experiment. C. elegans nasp-1 / btr-1 mutant versus N2, exposed to Bacillus thuringiensis DB27. 3 biological replicates, including 1 dye-swaps.
2013-01-31 | E-GEOD-43905 | biostudies-arrayexpress
Project description:Alkaline pH triggers an adaptation mechanism in fungi that is mediated by Rim101/PacCp, a zinc finger transcription factor. To identify the genes under its control in Ustilago maydis, we performed microarray analyses, comparing gene expression in a wild type strain vs a rim101/pacC mutant of the fungus. In this study we obtained evidence of the large number of genes regulated mostly directly, but also indirectly (probably through regulation of other transcription factors) by Rim101/PacCp, including proteins involved in a large number of physiological activities of the fungus. Our analyses suggest that the response to alkaline conditions under the control of the Pal/Rim pathway involves changes in the cell wall and plasma membrane through alterations in their lipid, protein, and polysaccharide composition, changes in cell polarity, actin cytoskeleton organization, and budding patterns. Also as expected, adaptation involves regulation by Rim101/PacC of genes involved in the meiotic functions, such as recombination and segregation, and expression of genes involved in ion and nutrient transport, as well as general vacuole functions. The mutant analyzed in this study is described in PMID 15947192
2014-07-21 | GSE53341 | GEO
Project description:Alkaline pH triggers an adaptation mechanism in fungi that is mediated by Rim101/PacCp, a zinc finger transcription factor. To identify the genes under its control in Ustilago maydis, we performed microarray analyses, comparing gene expression in a wild type strain vs a rim101/pacC mutant of the fungus. In this study we obtained evidence of the large number of genes regulated mostly directly, but also indirectly (probably through regulation of other transcription factors) by Rim101/PacCp, including proteins involved in a large number of physiological activities of the fungus. Our analyses suggest that the response to alkaline conditions under the control of the Pal/Rim pathway involves changes in the cell wall and plasma membrane through alterations in their lipid, protein, and polysaccharide composition, changes in cell polarity, actin cytoskeleton organization, and budding patterns. Also as expected, adaptation involves regulation by Rim101/PacC of genes involved in the meiotic functions, such as recombination and segregation, and expression of genes involved in ion and nutrient transport, as well as general vacuole functions. The mutant analyzed in this study is described in PMID 15947192 Five different oligonucleotide probes per gene (60 nt in length) per duplicate represented each of the 6,883 genes of the U.maydis genome. Accordingly, the expressed data are equivalent to 10 different assays of the expression of each gene.
2014-07-21 | E-GEOD-53341 | biostudies-arrayexpress
Project description:Staphylococcus aureus is a leading cause of hospital- and community-associated infections. The organism’s ability to cause disease can, in part, be attributable to its ability to adapt to otherwise deleterious host-associated stresses. Like other bacterial species, the modulation of mRNA turnover appears to play an important role in S. aureus adaptation to certain environmental stresses. In the current study Affymetrix GeneChips® were used to examine the S. aureus responses to acid and alkaline shock-inducing conditions and to assess whether stress dependent changes in mRNA turnover are likely to facilitate the organism’s ability to tolerate extreme pH challenge. Results indicate that S. aureus adapts to pH shock by eliciting responses expected of organisms coping with pH alteration, including neutralizing cellular internal pH, DNA repair, amino acid biosynthesis and virulence factor expression. Further, it was found that the cellular response to alkaline conditions elicits a transcriptional profile that is similar to that of stringent response induced cells. Consistent with that observation, we show that the activator of the stringent response, (p)ppGpp, levels are profoundly elevated during alkaline shock conditions. We also show that the mRNA turnover properties of acid or alkaline shocked cells significantly differ from that of cells grown at neutral pH. A comparison of the mRNA degradation properties of transcripts whose titers either increased or decreased in response to sudden pH change revealed that alterations in mRNA degradation may, in part, account for the changes in the mRNA levels of factors predicted to mediate pH tolerance. Finally, a set of small stable RNA molecules were induced in response to acid or alkaline shock conditions. As in other organisms, these molecules may mediate mRNA stability and adaptation to otherwise deleterious growth conditions. Staphylococcus aureus strain UAMS-1 was grown to exponential phase and either mock treated (pH maintained at 7.4) or subjected to acid (pH 4)- or alkaline (pH 10) conditions for 30 min. Next, 200 micrograms per ml of rifampicin were added to arrest transcript synthesis. RNA was extracted from cell suspensions at 0, 2.5, 5, 15, and 30 min post-transcriptional arrest, labeled and hybridized to S. aureus GeneChips. A comparison of 0 min samples allowed assessment of acid and alkaline shock responses. A comparison of 0 min to that of various post-transcriptional arrest RNA samples allowed assessment of the mRNA turnover properties of mock vs acid or alkaline shocked cells. Duplicates of each experimental condition and corresponding post-transcriptional arrest time point were used (biological replicates).
2010-10-01 | E-GEOD-22233 | biostudies-arrayexpress
Project description:Alkaline hemicellulytic bacteria Bacillus sp. N16-5 has abroad substrate spectrum and exhibits great growth ability on complex carbohydrates. In order to get insight into its carbohydrate utilization mechanism, global transcriptional profiles were separately determined for growth on glucose, fructose, mannose, galactose, arabinose, xylose, galactomannan, xylan, pectin and carboxymethyl cellulose by using one-color microarrays.
2014-06-17 | GSE38276 | GEO
Project description:To identify known and novel miRNAs involved in the response and adaptation of sugar beet to short-term and long-term alkaline stress, miRNAs were identified by analysis of the deep sequencing of sRNA
2021-02-28 | GSE113161 | GEO
Project description:Proteomics was employed to elucidate the intricate molecular mechanisms underlying the stress response of N. thermophilus when confronted with the synergistic challenges of high salt, alkaline pH, and elevated temperatures (HSAT conditions). This pioneering study presents the first comprehensive protein-level blueprint for polyextremophiles, uncovering a sophisticated interplay and synergy among halophilic, alkaliphilic, and thermophilic adaptive strategies. Our discoveries not only enrich the understanding of extremophile biology but also open up exciting avenues for exploring the resilience of life in extraterrestrial ecosystems, thereby offering invaluable prospects for astrobiological research.
2024-09-03 | PXD055526 |
Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.
2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress