Ontology highlight
ABSTRACT:
SUBMITTER: Yu J
PROVIDER: S-EPMC5356192 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Scientific reports 20170317
Forward genetic screens using retroviral (or transposon) gene-trap vectors in a haploid genome revolutionized the investigation of molecular networks in mammals. However, the sequencing data generated by Phenotypic interrogation followed by Tag sequencing (PhiT-seq) were not well characterized. The analysis of human and mouse haploid screens allowed us to describe PhiT-seq data and to define quality control steps. Moreover, we identified several blind spots in both haploid genomes where gene-tra ...[more]