Ontology highlight
ABSTRACT:
SUBMITTER: Schwarz H
PROVIDER: S-EPMC5357793 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Schwarz Harald H Gornicec Jan J Neuper Theresa T Parigiani Maria Alejandra MA Wallner Michael M Duschl Albert A Horejs-Hoeck Jutta J
Scientific reports 20170320
Low endotoxin recovery (LER) is a recently discovered phenomenon describing the inability of limulus amebocyte lysate (LAL)-based assays to detect lipopolysaccharide (LPS) because of a "masking effect" caused by chelators or detergents commonly used in buffer formulations for medical products and recombinant proteins. This study investigates the masking capacities of different buffer formulations and whether masked endotoxin is biologically active. We show that both naturally occurring endotoxin ...[more]