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Superficially porous particles with 1000A pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.

ABSTRACT: To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core®, core shell or porous shell) particles with very large (1000Å) pores specifically developed for separating very large biomolecules and polymers. Separations of DNA fragments, monoclonal antibodies, large proteins and large polystyrene standards are used to illustrate the utility of these particles for efficient, high-resolution applications.

SUBMITTER: Wagner BM 

PROVIDER: S-EPMC5360106 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.

Wagner Brian M BM   Schuster Stephanie A SA   Boyes Barry E BE   Shields Taylor J TJ   Miles William L WL   Haynes Mark J MJ   Moran Robert E RE   Kirkland Joseph J JJ   Schure Mark R MR  

Journal of chromatography. A 20170131

To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core<sup>®</sup>, core shell or porous shell) particles with very large (1000Å) pores specific  ...[more]

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