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MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/?-catenin signaling.

ABSTRACT: Accumulated evidence indicate that miR-744 functions as either tumor suppressor or oncogene in the progression of a variety of tumors, with a tumor type-specific way. However, little is known about how miR-744 impacts on the tumorigenesis of human prostate cancer. In this study, employing the analyses of microarray, qRT-PCR and re-analysis of MSKCC data, we found that CRPC tissues expressed much more miR-744 than ADPC tissues did, and the expression level of miR-744 was inversely associated with survival of CRPC patients. In vitro studies revealed that miR-744 promotes PCa cells proliferation, enhances migration, invasion; in vivo results demonstrated that silencing of miR-744 mediated by shRNA dramatically reduces PCa xenograft tumor growth. Importantly, through human gene expression array, pathway enrichment analysis and Western blot, we identified that miR-744 dramatically activated Wnt/?-catenin pathway by targeting multiple negative regulators of Wnt/?-catenin signaling, including SFRP1, GSK3?, TLE3 and NKD1. At molecular level, we further defined that NKD1 is a major functional target of miR-744. Our findings indicate that miR-744 acts as one of oncogenic factor in the progression of CRPC by recruiting a mechanism of aberrant activation of Wnt/?-catenin signaling.

SUBMITTER: Guan H

PROVIDER: S-EPMC5362436 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.

Guan Han H Liu Chunhui C Fang Fang F Huang Yeqing Y Tao Tao T Ling Zhixin Z You Zonghao Z Han Xu X Chen Shuqiu S Xu Bin B Chen Ming M

Oncotarget 20170201 9

Accumulated evidence indicate that miR-744 functions as either tumor suppressor or oncogene in the progression of a variety of tumors, with a tumor type-specific way. However, little is known about how miR-744 impacts on the tumorigenesis of human prostate cancer. In this study, employing the analyses of microarray, qRT-PCR and re-analysis of MSKCC data, we found that CRPC tissues expressed much more miR-744 than ADPC tissues did, and the expression level of miR-744 was inversely associated with ...[more]

PMID: 28107193

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Dataset Information

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/?-catenin signaling.

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MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.

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