Ontology highlight
ABSTRACT:
SUBMITTER: Knipping F
PROVIDER: S-EPMC5363317 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Knipping Friederike F Osborn Mark J MJ Petri Karl K Tolar Jakub J Glimm Hanno H von Kalle Christof C Schmidt Manfred M Gabriel Richard R
Molecular therapy. Methods & clinical development 20170212
In T cells with transgenic high-avidity T cell receptors (TCRs), endogenous and transferred TCR chains compete for surface expression and may pair inappropriately, potentially causing autoimmunity. To knock out endogenous TCR expression, we assembled 12 transcription activator-like effector nucleases (TALENs) and five guide RNAs (gRNAs) from the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Using TALEN mRNA, TCR knockout was successful in up ...[more]