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Halo-free Phase Contrast Microscopy.

ABSTRACT: We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.

SUBMITTER: Nguyen TH

PROVIDER: S-EPMC5364506 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Halo-free Phase Contrast Microscopy.

Nguyen Tan H TH Kandel Mikhail M Shakir Haadi M HM Best-Popescu Catherine C Arikkath Jyothi J Do Minh N MN Popescu Gabriel G

Scientific reports 20170324

We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo ...[more]

PMID: 28338086

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