Ontology highlight
ABSTRACT:
SUBMITTER: Stachura M
PROVIDER: S-EPMC5372835 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Stachura Monika M Chakraborty Saumen S Gottberg Alexander A Ruckthong Leela L Pecoraro Vincent L VL Hemmingsen Lars L
Journal of the American Chemical Society 20161220 1
Nanosecond ligand exchange dynamics at metal sites within proteins is essential in catalysis, metal ion transport, and regulatory metallobiochemistry. Herein we present direct observation of the exchange dynamics of water at a Cd<sup>2+</sup> binding site within two de novo designed metalloprotein constructs using <sup>111m</sup>Cd perturbed angular correlation (PAC) of γ-rays and <sup>113</sup>Cd NMR spectroscopy. The residence time of the Cd<sup>2+</sup>-bound water molecule is tens of nanosec ...[more]