Dataset Information

Genomic variants at 20p11 associated with body fat mass in the European population.

ABSTRACT:

Objective

Body fat mass (BFM) is more homogeneous and accurate than body total mass in measuring obesity but has rarely been studied. Aiming to uncover the genetic basis of fat-induced obesity, a genome-wide association meta-analysis of BFM, after adjustment by body lean mass, was performed in the European population.

Methods

Three samples of European ancestry were included in the meta-analysis: the Framingham Heart Study (N?=?6,004), the Kansas City osteoporosis study (N?=?2,207), and the Omaha osteoporosis study (N?=?968).

Results

At the genome-wide significance level (??=?5.0×10^-8 ), a cluster of 10 single-nucleotide polymorphisms (SNPs) at chromosomal region 20p11 that were associated with BFM (lead SNP rs2069126, P?=?1.82×10^-9 , closest gene SLC24A3) was identified in 9,179 subjects. One of the top SNPs, rs6046308 (P?=?3.74×10^-8 ), was found to be nominally significant for body fat percentage in another independent study (P = 0.03, N?=?75,888) and was reported to transregulate the expression of the MPZ gene at 1q23.3 (unadjusted P?=?9.78×10^-6 , N?=?1,490). Differential gene expression analysis demonstrated that SLC24A3 and CFAP61 at the identified locus were differentially expressed in tissues of people with versus without obesity (P = 3.40×10^-5 and 8.72×10^-4 , N?=?126 and 70), implying their potential role in fat development.

Conclusions

These results may provide new insights into the biological mechanism that underlies fat-induced obesity pathology.

SUBMITTER: Pei YF

PROVIDER: S-EPMC5373963 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Publications

Genomic variants at 20p11 associated with body fat mass in the European population.

Pei Yu-Fang YF Ren Hai-Gang HG Liu Lu L Li Xiao X Fang Chen C Huang Yun Y Hu Wen-Zhu WZ Kong Wei-Wen WW Feng An-Ping AP You Xin-Yi XY Zhao Wen W Shen Hui H Tian Qing Q Zhang Yong-Hong YH Deng Hong-Wen HW Zhang Lei L

Obesity (Silver Spring, Md.) 20170222 4

<h4>Objective</h4>Body fat mass (BFM) is more homogeneous and accurate than body total mass in measuring obesity but has rarely been studied. Aiming to uncover the genetic basis of fat-induced obesity, a genome-wide association meta-analysis of BFM, after adjustment by body lean mass, was performed in the European population.<h4>Methods</h4>Three samples of European ancestry were included in the meta-analysis: the Framingham Heart Study (N = 6,004), the Kansas City osteoporosis study (N = 2,207) ...[more]

PMID: 28224759

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Project description:BACKGROUND:The risk of obesity is determined by complex interactions between genetic and environmental factors. Little research to date has investigated the interaction between gene and food intake. The aim of the current study is to explore the potential effect of fat mass and obesity-associated protein gene (FTO) rs9939609 and rs9930506 single nucleotide polymorphism (SNP) on the pattern of food intake in the Emirati population. METHODS:Adult healthy Emirati subjects with Body mass index (BMI) of 16-40 kg/m2 were included in the study. Genotyping for FTO rs9939609(A>T) and rs9930506(A>G) was performed using DNA from saliva samples. Subjects were categorized according to the WHO classification by calculating the BMI to compare different classes. Dietary intake was assessed by a sixty-one-item FFQ that estimated food and beverage intakes over the past year. The daily energy, macronutrient, and micronutrient consumption were computed. RESULTS:We included 169 subjects in the final analysis (mean age 30.49± 9.1years, 57.4% females). The mean BMI of the study population was 26.19 kg/m2. Both SNPs were in Hardy Weinberg Equilibrium. The rs9939609 AA genotype was significantly associated with higher BMI (p = 0.004); the effect was significant in females (p = 0.028), but not in males (p = 0.184). Carbohydrate intake was significantly higher in AA subjects with a trend of lower fat intake compared to other genotypes. The odds ratio for the AA was 3.78 in the fourth quartile and 2.67 for the A/T in the second quartile of total carbohydrate intake, considering the first quartile as a reference (95% CI = 1.017-14.1 and 1.03-6.88, respectively). Fat intake was significantly lower in the FTO rs9930506 GG subjects. The presence of FTO rs9930506 GG genotype decreased the fat intake in subjects with FTO rs9939609 AA (p = 0.037). CONCLUSIONS:The results of this study highlight the interaction of the FTO risk alleles on the food intake in Emirati subjects. The FTO rs9939609 AA subjects had higher carbohydrate and lower fat intake. The latter was accentuated in presence of rs9930506 GG genotype.

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Dataset Information

Genomic variants at 20p11 associated with body fat mass in the European population.

Objective

Methods

Results

Conclusions

Publications

Genomic variants at 20p11 associated with body fat mass in the European population.

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