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Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput.

ABSTRACT: Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

SUBMITTER: Gierahn TM

PROVIDER: S-EPMC5376227 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput.

Gierahn Todd M TM Wadsworth Marc H MH Hughes Travis K TK Bryson Bryan D BD Butler Andrew A Satija Rahul R Fortune Sarah S Love J Christopher JC Shalek Alex K AK

Nature methods 20170213 4

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosi ...[more]

PMID: 28192419

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